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17 February 2021 4,217 3 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

16 February 2021 8,344 2 View

Why do cells die after adding MTT dye?

I was doing MTT assay, and in the last day before adding the MTT; the cells looked good (or so I think), but after aspiration of the drug and addition of MTT dye I noticed that most cells seemed...

07 February 2021 3,846 4 View

How do I get spectral power distribution (SPD) of my photoluminescence sample to calculate chromaticity coordinates?

Hi, I have photoluminescence samples which I already characterized and from that I already obtained the steady-state photoluminescence (SSPL) property. I want to draw CIE chromaticity diagram to...

24 January 2021 7,770 1 View

How can I stain 3d printed cells in hydrogel without damaging the gel?

I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...

17 January 2021 4,170 1 View

How can one synthesize the Inverted Core-Shell Particles (ICS)?

Dear RG colleagues, how can one synthesize the inverted core-shell particles (ICS) from a polymer and a Nano-metal oxide? Where the polymer is a core and the metal oxide is a shell. Under which...

01 December 2020 9,895 2 View

Why do I get low absorbance in MTT assay?

I have been trying to do MTT assay using HCT-116 cells but I have been getting very low absorbance readings (absorbance is usually 0.25 and below) even though iam seeding 30,000 cell/ well. I have...

16 November 2020 3,588 3 View

What do you think the percentage of educational lost by students in courses where labs and workshops are necessary?

During the current pandemic, most educational institutions all over the world were forced to on-line education which led students to be away from practice applications such as labs and workshops....

30 October 2020 3,254 16 View

What is the relationship (equation) between node transmission range and node density in wsn?

I am simulating routing protocol in wsn the orginal simulation was based on 802.11 mac and physical layer and I am using 802.15.4 as you know the transmission range in 802.15.4 is less, which...

20 August 2020 8,888 2 View

Are you interested in IEEE & Scopus Indexed Conference ?

Dear Colleagues, I am pleased to announce the 3ICT 2020 conference where you can publish your research finding without travelling. All papers registered and presented at 3ICT2020 conference will...

23 July 2020 4,077 2 View

Do you need to immortalize primary sarcoma cells to establish a cell line?

We had recently isolated primary sarcoma cells from a patient. We are looking to establish a cell line from these cells using HTERT gene insertion.

31 December 2019 9,049 1 View

Which cell line to use for checking the over-expression of MDM2 ?

I found no consensus on the use of a particular cell line as the mdm2 gene expression is non-specific.

09 October 2019 5,115 2 View

Is it necessary to remove restriction enzymes from a restriction digest prior to transfection?

I'm trying to design an experiment to transfect Ewing Sarcoma cells with a plasmid carrying a targeted fluorophore. Since this is my first time trying to design an experiment like this, I was...

05 February 2019 8,798 5 View

Is it necessary to remove restriction enzymes from a restriction digest prior to transfection?

I'm trying to design an experiment to transfect Ewing Sarcoma cells with a plasmid carrying a targeted fluorophore. Since this is my first time trying to design an experiment like this, I was...

01 February 2019 6,693 6 View

Which primers should I use for WT TP53 (qPCR experiment)?

Hi everyone, I am a student fairly new to experimental research! I am looking to do qPCR on different sarcoma samples to confirm the presence of TP53 (17p13.1). Since I am not seeking the presence...

11 December 2018 7,043 3 View

Which primers should I use for WT TP53 (qPCR experiment)?

Hi everyone, I am a student fairly new to experimental research! I am looking to do qPCR on different sarcoma samples to confirm the presence of TP53 (17p13.1). Since I am not seeking the presence...

10 December 2018 3,810 5 View

What is the optimum dimensions for interdigitated amperometric biosensor?

what is the optimum dimensions for interdigitated amperometric Label-free biosensor( Length, width and separation) for detect protein bio-marker ? also what is the best chose for working...

09 October 2018 3,770 2 View

Willing to share human tumor dissociation protocols?

Hi, Thank you for reading my question. I would be interested in protocol available to dissociate human sarcoma samples, solid and not. On more general field, I would love to read your protocols...

07 August 2018 1,860 0 View

CAN YOU HELP ME DO A RESEARCH ON FOOD SOUVENIRS BY ANSWERING A TWO-MINUTE QUESTIONNAIRE?

I am trying to conduct a pilot survey on food souvenirs. Please help me by answering to a few questions in the Questionnaire on the following link: https://www.surveymonkey.com/r/JWWQ9ZP Thank...

11 December 2017 5,464 21 View

I want to know type of DMEM media with soft tissue sarcoma

high or low glucose DMEM media with soft tissue sarcoma

04 May 2017 5,733 1 View