How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

How to do Statictical analysis for unequal sample sizes?

Hi Everyone, I have a dataset from proteomics analysis of treated cancer cell line, I have three replicates in treatment time-points and two replicate in control condition. Now, How can I...

01 January 2021 4,055 3 View

How can I dry aluminum flake without the danger of ignition?

In order to produce Al flake in the ball mill, it is kept under an organic solvent. how can we remove it later from this solvent?

06 July 2020 6,232 7 View

Has anyone ever taken crystal of meta boric acid or B NMR ?

I'm making a boron-containing by-product which in only soluble in DMSO. I've been trying to make a crystal of that. I'm kind of sure is meta boric acid and I have a peak at around 20 ppm in B NMR.

05 February 2020 2,197 2 View

Which additive can create tone (especially green) in white ABS masterbatches to omit the undesirable yellow color ?

white ABS masterbatch usually has bluish or greenish color. optical agents lead to green tone in white masterbatch and omit inherent yellow color of ABS but will decompose during the process.

11 October 2019 3,740 1 View

How to choose the proper neural network Classifier in MATLAB?

I want to use a neural network classifier to separate patients and healthy persons by some parameters. and then test validation of the classifier. Is svm a proper tool? And how can I test the...

21 October 2018 8,968 8 View

Dose anyone know why ht29/5 fu resistant cells don't grow?

17 November 2016 6,149 3 View

How can I solve this RACE PCR problem to make the band visible?

01 January 2015 8,545 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How do you write a simplified C block code of this average-current control on PSIM?

This is a Buck (step-down) Converter and I am new to this program. I need to program with simplified C block and I actually don't know what to do. Can anyone show me how to do this? Or at least...

02 March 2021 8,233 1 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

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25 February 2021 5,713 1 View