How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,351 2 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

Gain and offset values in Sentinel-2 L1C?

Hello everyone. I have been trying to apply radiometric calibration on Sentinel-2 L1C images (bands 2,3,4 and 8), but every time after layerstacking I come across this error: "Calibration...

28 January 2021 7,160 1 View

How to fix the voids on a floodplain map by using HEC_RAS model?

24 January 2021 9,054 2 View

How the precipitation Ratio and flow Ratio effect the output of the result?

I am doing HEC HMS calibration than i want to increase simulated value and volume of the computed so that my issue is how the precipitation and flow ratio make influential that result?

22 January 2021 152 2 View

Can anyone please tell me, what will be the Crop Coefficient value for shortleaf pine and oak tree in HEC-HMS model simulation?

my study area located in the forest region having Shortleaf Pine and Oaktree, thus in the Canopy Method I am trying to get the Crop Coefficient value of these trees but did not get anything.

18 January 2021 9,789 1 View

How can you confirm new liquid FTIR cell fits calibration?

We have an FTIR instrument which was calibrated in transmission/absorption mode using a set of cells for different ranges of D2O concentration. One of the cells at high range broke and the new one...

16 January 2021 4,791 2 View

Standard addition calibration without blank matrix available?

I have a problem of analytical chemistry. We are working on a spectroscopy technique (FTIR) for the quantification of a mineral phase in complex matrices. We intended to use chemometric techniques...

12 January 2021 7,739 1 View