I need to do cell viability assay in an experiment and I want to know the plating density and incubation time for the alamarBlue assay for human PBMC.
I used MT4 cells previously and had used 10,000 cells per well in 96 well plate and after adding alamar blue, incubation for 1-3 hr gave good results. Now I want to do the same using human PBMC (peripheral blood mononuclear cells). Please let me know the same if you have used human PBMC anytime. I am using Cat# DAL1100 AlamarBlue® Cell Viability Reagent . Thank you in advance. :)
hello,
I have used alamar blue assay several times for my experiments. but i found that there is some problem in this assay when we are doing time kinetics study. Its better to use MTT or trypan blue assay.
Best of luck
Hina
Thanks for your inputs. But, Alamar blue works fine and has got lots of advantages. For now, with PBMC it worked well for 10000 cells and 24 to 72 h of incubation. The best part is we don't have to go for the lysis of the cells after incubation and can take readings at multiple time points.
And best of luck to you too.
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