Think
Chloroform does not belong on a list of solvents that are generally considered safe. Its use has been widely discontinued because of its toxicity. On this list it can be replaced with propylene glycol, commonly used in foods and flavors.
My best option is Ethanol.
Both are Safe, and widely used for herbal and food extractions.
What food do intestinal bacteria like most?ChatGPTIntestinal bacteria love fibrous foods that are prebiotic. These foods include vegetables (such as broccoli, cabbage, and brussels sprouts),...
22 June 2024 4,567 0 View
I'm doing T7E1 experiment to check CRISPR-Cas9 transfection efficiency. Although i see my PCR products in the true site on the agarose gel, i do not see any band when i do T7E1. It is as if no...
29 November 2023 817 3 View
Can the stickiness of body secretions increase with the increase of gluten? Was gluten found in sputum?
19 June 2023 905 0 View
I made such an assumption because of the high amount of saponin in alfalfa. How true? Sincerely,
18 June 2023 7,231 1 View
This is a very technical curiosity. There is very mixed information about the ancient period, especially about the first civilizations. If this information is put in order with artificial...
30 May 2023 1,888 9 View
As known lithium chloride (LiCl) is inhibitor of GSK3 beta and which binds to GSK3 beta active site instead of Mg++. So, we found lithium perchlorate from another lab and can i see same effect of...
07 January 2021 6,258 2 View
Hi All, Our study is about miRNA function. In miRNAs biogenesis, argounate proteins provide 3p or 5p selection of miRNAs with thermodynamic stability. What is this ''Thermodynamic Stability''?...
10 May 2020 3,629 0 View
I am using 293T-BFP reporter cell. I am doing gene editing with Cas9. 293T-BFP cells have repair template plasmid and when cas9 cuts BFP site on template, BFP converts GFP by adding donor...
15 September 2017 5,775 2 View
Motivation and The Candle Problem by Rhoads on August 13, 2013 in Motivation Our world continues to move faster and is increasing in complexity. So what’s the best way to motivate people to think...
01 February 2016 299 22 View
Dear Colleagues, Under the auspices of the Rector of Tekirdağ Namık Kemal University Prof.Dr. Mümin Şahin, "Tekirdağ Namık Kemal University Faculty of Economics and Administrative Sciences...
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Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
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I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I need to do some experiment with it in liquid form.
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Palmitic acid presence in aqueous fraction
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I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
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HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
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I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View