Ensure that the dairy does not overwhelm the nutritional needs of the rat (Check the Nutritional Balance, avoid Excess Sugars and monitor any digestive issues).
Rats should not be given large amounts of dairy. A typical portion size could be about 1-2 teaspoons per rat per day, but this depends on the specific dietary requirements of your experiment.
Mixing with Regular Rat Diet :
Dairy products can be mixed into the rats' regular pelleted rat diet. You can crumble or mix small portions of cheese or yogurt into their standard diet.
If you are offering milk, consider adding it to their drinking water in small amounts. However, it should not replace their regular water, and rats should always have access to fresh water.
If adding dairy to a mash or wet food mix, ensure the mixture is thoroughly homogenized and does not spoil quickly.
Thank you Dr. Nicolas for your participation. I would like to ask another question: Can I freeze dry the milk or yogurt, then grind them and add them to the dry feed? I think that will reduce the chance of spoilage? Or is it better to add them in their original form?
Yes, freeze-drying milk or yogurt and then grinding it into a powder is an excellent way to preserve the nutrients and reduce the risk of spoilage. This approach is effective for reduced spoilage risk, easier storage, controlled portions, maintains nutritional content.
If your rats are lactose intolerant, freeze-drying won’t remove the lactose, so you’d need to choose lactose-free milk or yogurt for freeze-drying.
After freeze-drying and grinding, make sure the powder is fine enough to mix easily into the regular rat diet. You don’t want large clumps that might not mix evenly or get consumed properly.
While freeze-drying preserves nutrients well, verify the powder’s nutritional profile after the process, especially if you're using dairy as a significant supplement.
Even freeze-dried dairy should be stored properly in airtight containers to prevent any moisture from getting in, which could cause spoilage or clumping.
It will be interesting to compare your results from freeze-dried products with those from fresh dairy products to validate your protocol. Keep in mind that the microbiota of unpasteurized dairy products may be lost during the freeze-drying process.