I want to formulate TBST for western blot. But I don't know which specific formula is more suitable. How should I choose?
I would suggest buying the concentrated TBS, so all you have to do is dilute it and add the tween 20.
For PBS and TBS... I won't make it from scratch.
https://www.abcam.com/protocols/buffer-and-stock-solutions-for-western-blot#8
http://www.bio-rad.com/webroot/web/pdf/lsr/literature/Bulletin_6376.pdf
I always use
TBS-T (20 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% Tween 20)
It is actually Tris-buffered saline containing 0.1% Tween 20.
You can also go through our published article
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