I have transfected GFP-tag plasmid to THP-1 cells, and hope to check the efficiency. I used 24-well and there were 5*10^5 cells per well. I used 50ul opti-MEM mixed with 1ul lipo, and used another 50ul opti-MEM mixed with my plasmid.
24 hours later, I checked the cells by fluorescence microscope. There were approximately 20 cells with fluorescence each well. The efficiency was about 1 to 10000.
Although the suspension cells have a low efficiency in transfection, but I am wondering if it is normal or not? If not, I have to check my technology of transfection.