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Questions related from Miao Lee
Hello, I am puzzled with my protein immunoblotting. I have constructed a plasmid pcDNA3.1(+) concluding my target gene fusion with FLAG tag on its N-terminal sequence. We got the plasmid sequence...
02 July 2019 5,134 3 View
Hello everyone, I have had two same clones by using different cDNA from different cells. Before the sequencing, I got single clone of DH5-a bacteria and have PCR, restriction enzyme to confirm...
11 May 2015 7,148 3 View
I am doing research on autophagy with bacteria. According to our lab protocol, first we leave the cells (RAW264.7) infected with bacteria for an hour at MOI=10, then wash out the cells in order to...
02 April 2014 10,020 2 View
I have got some IF results using LSM, and I got the merge results on that computer. However, what software can I use to conform all the result and get a total result in one picture? Can someone...
16 March 2014 4,561 8 View
I have used THP-1 and RAW cell line to confirm a result, however, the results differ from each other. Is this because that my THP-1 cell line was not derived from monocytes to macrophages?
19 January 2014 3,981 3 View
I have transfected GFP-tag plasmid to THP-1 cells, and hope to check the efficiency. I used 24-well and there were 5*10^5 cells per well. I used 50ul opti-MEM mixed with 1ul lipo, and used another...
02 January 2014 633 3 View
Now, i am going to find the ligand of molecular X. We are preparing to overexpress the molecular X in cells , and get the cell membrane to co-IP. But someone has told me the ligand and the...
02 June 2013 8,217 10 View
I want to do the plasmid midi research by 25ml DH5α-plasmid, but the concentration i got is very low, about 10 ng/ul. I followed the protocol every step, and i don't know the reason. Can i...
09 May 2013 6,353 3 View
