Why DMEM color is changed?

Why the pink color will change to yellow, when the DMEM media is frozen? however after dissolution in room temperature, media color will change back into pink.

26 October 2020 708 2 View

A genome wide CRISPR/Cas9 screen for drug resistance in cancer cells?

03 November 2019 7,662 2 View

What kinds of single indicators are appropriate, when we calculate multifunctionality?

A (wide accepted) view is that, when we try to address on the biodiversity-ecosystem function relationships, the multifunctionality index should be calculated by a set of activity indicators,...

02 September 2019 8,370 2 View

How to choose solvent C18 column for separating polar compounds?

I have one polar product with an impurity, which has the similar structure and polarity. I used the Hexane:Ethyl acetate:Methanol in 6:3:1with silical gel. And the difference of Rf is around 0.08,...

06 August 2019 3,572 2 View

How to fabricate/make silica spin column for DNA/RNA extraction?

Dear Friends, Does anyone know how to fabricate/make silica spin column for DNA/RNA extraction? Any books, references and videos are welcome. Thanks in advance. GH Yue

27 June 2019 2,456 3 View

How to present the complete results of stepwise regression?

The common form is the presentation of regression equation, with the coefficients of determination and p value included in the brackets. But we know that the beta coefficients and sum of squares...

02 March 2019 6,064 6 View

Do you have information of extremely stable DNA?

Dear friends, I am looking some extremely stable DNA sequences. If you have some info on this, please let me know. Regards, Gen Hua

24 June 2018 591 2 View

Does anyone know if there is a method for the complex CRISPR/dCas9 to become fluorescent only when it binds to the gDNA?

Hi, I saw that it is possible to target and image a single locus on the genomic DNA with a cocktail of CRISPR/dCas9 following the CasFISH method. However, the signal is quite diffuse and I want to...

15 June 2018 1,583 2 View

Can RNA shuttle between the nucleus and cytoplasm?

Hi, everyone As we all know, a protein can shuttle between the nucleus and the cytoplasm. Whereas many proteins are selectively transported from the cytoplasm into the nucleus, most RNAs are...

10 April 2018 9,318 3 View

What is the distribution of most frequently outcome when sampling from a multinomial distribution ?

08 April 2018 1,890 5 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View

When to use different framework?

Hi, can I use both conceptual framework and theoretical framework in my systematic review? My future study plan is to use a theory. In this situation for my systematic review should I use both or...

18 February 2021 9,581 2 View

Why use both bead beating and enzymes (lysostaphin and lysozyme) for DNA extraction from sputum samples?

Hello, I have read in a few publications that to extract total DNA from sputum samples, both bead beating and enzymes are used to disrupt the cells. Bead beating is supposed to be efficient in...

18 February 2021 3,360 2 View

Which test should I use in SPSS if I have two independent variables and one dependent variable that is measured using two tests?

I am researching the effect of gender and video game use on reaction times. To do this, I will have participants complete two reaction time tests (a simple test and a four choice test). I am a...

17 February 2021 9,343 4 View

How to judge and prove the uniqueness of the solutions to the following two matrix equations?

It is about the matrix equation AX=B, where a, b c in A and B are independent elements, X should be made of constants. Case 1 A=[a b 0;0 0 c]; B=[a b 0 0;0 0 c c]; The solution is X=[1 0 0 0;0 1...

16 February 2021 5,698 3 View

Which DL architecture is better to work on handwritten data?

I am working in the area of Handwriting recognition for Bangla. As there are many DL architecture, I am confused which one to select.

15 February 2021 4,996 1 View

How to obtain Contig sequence from BAM file?

I have use HISAT2 for alignment . Now i want to obtain contig sequence from from BAM file in galaxy server? I have used Samtools View. That is giving me some sequence but I am not sure whether it...

12 February 2021 3,262 4 View