I would like to ask about the depth of analysis for producing a paper on identification of species using DNA barcoding technique. For example, I have sampled 3 species of spiders in cave and extracted their gDNA, design COI primers, run PCR and managed to obtain their respective barcodes for species identification. The similarity precentage of all the sequences was between 99-100%. If the species could be determined after BLASTn search, do I still need to proceed for phylogenetic analysis or other relevant analysis? Is the data enough to produce a paper because many papers that I have referred include a number of in depth analysis. Please enlighten on this. Thank you.
The fewer data you can use, the better; then your paper will be more elegant and enlightened :)
Oh thank you Andrew. But is it enough to fullfill the depth of analysis? Since there were only three samples, not much analysis I can perform apart from COI sequence recovery and phylogenetic analysis.
The depth and amount of analysis depends on amount of questions, which you want to find answers to in your work. For instance, three individuals is definitely not enough to say something about population structure, but enough to determine the species.
Hi Kostin, the aim of the study is to determine the species. I appreciate your opinion and now I can proceed with the paper. Thanks once again!
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