The Conditions When Gram Positive Bacteria Can Appear Gram Negative?
- When over-decolourized by either prolonged exposure to decolourizer or using acetone alone.
- When cell wall gets damaged by exposure to lysozyme or cell wall acting antibiotics such as Penicillin.
- Old cultures, where cell wall is weakened or action of autolytic enzymes.
- Those bacteria that are phagocytosed. where cell wall is acted upon by lysosomal contents.
If the decolorizing agent is applied to the cell for too long a time, the Gram-positive organisms appear Gram-negative. Under-decolorization occurs when the alcohol is not left on long enough to wash out the CV-I complex from the Gram-negative cells, resulting in Gram-negative bacteria appearing Gram-positive.
https://www.ssajm.org/article.asp?issn=2384-5147;year=2014;volume=1;issue=4;spage=168;epage=174;aulast=Thairu
Gram-positive bacteria will sometimes lose the ability to hold the stain that makes us call it gram positive and it will appear gram negative. Older cultures of certain gram-positive organisms may lose their ability to hold the stain.
https://www.quora.com/What-are-the-conditions-when-gram-positive-bacteria-can-appear-gram-negative-bacteria
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