Literature Review for a dissertation- any tips?

Hello everyone I hope you are all well! I just have a quick question- I am a third year BA Social Work student in the midst of writing a literature review for my dissertation. I wanted to know if...

04 January 2021 6,869 4 View

Oocyte cultivation media - where can I find out about them?

Hello, can someone help me? I am just random undergrad interested in oocytes and early embryos but I know almost nothing about their in vitro cultivation. What is the current state of the art of...

23 August 2020 1,817 1 View

Can I pipette my small cDNA amount touching the wall of the tube? Can I sterilise my plastic well-plate by UV before using?

Hi, I have some questions about qPCR mix preparation. 1) If I am pipeting a small volume (0,5 microliter) of my cDNA after adding master mix, I draw off the liquid to the wall of the PCR well, not...

03 August 2020 3,940 4 View

What changes do you expect in GMO regulation in the context of clima change (esp. in european legal context)?

GMO is, among other uses, a technology that may enable us to better utilize natural sources (both in agriculture and in industry, tec.). However, its practical utilization is strictly controlled,...

26 March 2020 3,767 3 View

What plastic bag to use for soil gamma irradiation?

I plan to process soil samples with gamma irradiation. Obviously, they have to be kept in airtight plastic bags for no later air exchange and possible contamination. I was initially considering...

06 October 2019 9,097 2 View

FTA cards : can be use with DNA extraction directly ?

Hello everyone, I need to know if we can use FTA cards with DNA extract obtained with kit or with the phenol chloroform method ? ? Or if its just for biological samples ? Because, I want to do...

23 July 2018 142 3 View

Do you have similar experience with pitfalls of minigene splicing assays ?

Minigene splicing assay is undoubtedly useful and popular method to dissect splicing regulation, its aberrations, etc. When used for detecting the effect of mutations on splicing, most of its...

24 August 2015 6,149 3 View

Searching for people to share information about wild plant uses in California, Chile and Malaysia.

I'm travelling 8 months through California (sept.-november), Chile (Aysen Region) (november-january) and Malaysia (february-april) to learn about uses for wild plants. In France, I used to teach...

16 September 2013 9,142 4 View

What does it really mean "in vivo"?

I was taught that "in vivo" experiment means an experiment undergone in a living organism. However, in many publications, I find the term "in vivo" used for the experiments done on cell cultures....

27 August 2013 4,543 38 View

Does anyone have any experience with the pcDNA3-mRFP plasmid?

I am searching for a plasmid expressing RFP in human cell lines. The RFP should work as a transfection control in flow cytometry experiments. Since I prefer some low-cost solution, I found...

02 June 2013 913 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View