Hello,

I am working with chicken primary muscle cells that I tried to isolate from fresh chicken breast, however, several of my samples were infected, and I have no clue what it is, and also how to treat it. I tried several recommendations like leaving cells 10 mins in PBS with ATB or increasing slightly concentration of ATB in media. Unfortunately, nothing helped, and I have infected most of my samples, even though I tried to treat them separately for washing, passaging, and changing media, to be sure that I don't spread it to others. Of course, I tried to cultivate media and reagents separately for several days to see if there are ok, so it wasn't a problem. I would be happy if someone has similar experience or suggestions for solutions:) Thank you!