8 Questions 18 Answers 0 Followers
Questions related from Lucie Grodecká
Dear researchers, a variability in the taxonomy classification of microbial communities when using different primer pairs (e.g. for 16S rDNA) is commonly known. However, the mismatches to these...
18 March 2024 8,160 1 View
What is the best way of metagenomic analysis using multiple rDNA amplicons (e.g. multiple 18S and ITS for each sample)? What methodology would you use to reliably normalize and integrate the data...
22 November 2023 8,841 3 View
GMO is, among other uses, a technology that may enable us to better utilize natural sources (both in agriculture and in industry, tec.). However, its practical utilization is strictly controlled,...
27 March 2020 3,891 3 View
Hi, could you recommend me where to obtain the best vector constructs for SRSF1, hnRNPA1 and U2AF35 overexpression in HeLa cells? Preferentially without any tag. Thank you!
26 July 2018 5,988 1 View
Hi, please, do you know about any publication that sufficiently explains the mechanism of danazol effect on HAE patients? Thank you in advance. Lucie
01 September 2016 9,433 7 View
Minigene splicing assay is undoubtedly useful and popular method to dissect splicing regulation, its aberrations, etc. When used for detecting the effect of mutations on splicing, most of its...
25 August 2015 6,252 3 View
I was taught that "in vivo" experiment means an experiment undergone in a living organism. However, in many publications, I find the term "in vivo" used for the experiments done on cell cultures....
28 August 2013 4,631 38 View
I am searching for a plasmid expressing RFP in human cell lines. The RFP should work as a transfection control in flow cytometry experiments. Since I prefer some low-cost solution, I found...
03 June 2013 998 3 View
