The best study of Zoom use during pandemic

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Has anyone use Emx1Cre adult mice to label pyramidal neurons?

I want to inject a Cre dependent virus to be expressed in pyramidal neurons, and I have Emx1Cre adult mice. I know Emx1 is a developmental gene, but found a paper showing expression of Emx1 also...

28 March 2024 1,666 0 View

Genotyping plants for overexpression insertion?

I have plants (L. japonicus) that have been transformed with an overexpression plasmid. How can I know that these plants are homozygous for the insertion?

24 March 2024 8,533 2 View

Are there any guidelines for using CHAT GPT in your research paper title?

I always take the longest time thinking about titles and writing abstracts, so I think it might be a good use. But I wonder if journals should include this in their guidelines, or else, it's not a...

12 February 2024 7,730 1 View

OMVs labeling for uptake assay?

Good evening, Can you please advice on a protocol for labeling of OMVs for cellular uptake assay. OMVs are derived from gram-negative strain. I was thinking of applying R-18 but other dyes are...

03 October 2023 2,929 3 View

Problem with F-AAS Zinc quality controls standards?

Hello, in our lab we use F-AAS for determination of Mg in urine and Li, Zn in serum. Except Zinc QC 2 everything works fine. Is it possible there is problem with zinc controls? I am waiting for...

04 September 2023 4,169 1 View

Does anyone has a CamKII primary antibody to recommend for immunostaining in slices?

Hi! Im experiencing problems with CamKII immunostaining for free floating mouse PFC and Hp slices. I ve tried different blocking agents ( NDS, NGS, BSA in different percentages), different mouse...

30 July 2023 7,341 0 View

How to CRISPR-KO and CRISPR-HDR of p53 in fibroblasts?

I performed CRISPR-KO of p53 in fibroblasts. I've got cells that lost the activity of WTp53 (several target genes tested on qPCR) but on western blot, I see still quite a high level of a protein...

15 January 2023 8,080 0 View

ImageJ or QuPath?

How can I quantify DAB using ImageJ or QuPath (staining intensity and number of positive cells)?

03 July 2022 6,002 3 View

ReproSil NH2 column for sugars analysis - mobile phase?

I have five different columns from Dr.Maish: 1) ReproSil - XR 120 NH2 2) Reprosil 80 NH2 3) Reprospher 100 CN 4) Reprosil - Pur 120 NH2 5) Reprosil Saphir 100 NH2 4) Reprosil - Pur 120 NH2 Can I...

08 June 2022 2,467 0 View

What polymeric material have the most similar properties as nylon 6.6 , and it is not from the polyamides family?

What polymeric material has the most similar properties as nylon 6.6 in terms of the processing and mechanical properties, and it is not from the polyamides family?

05 August 2021 6,193 8 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How to get links for copyrights for papers?

how to get links for copyrights for papers?

06 August 2024 7,410 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Is there a TPACK assessment tool on utilization of science learning tools and equipment?

TPACK Technological, Pedagogical, and Content Knowledge

03 August 2024 740 3 View

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

What do you consider to be the most relevant elements of EEG for studying cognitive biases?

I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...

31 July 2024 7,259 1 View

Any tips on how to increase the chances of getting a response?

I've been using ResearchGate for a while now and have found many useful research papers that are beneficial for my work. However, I've noticed that many authors do not respond to my messages....

31 July 2024 7,771 5 View

I have no added any resarch paper yet but showing three paper? how to delete it?

I have not addede any paper yet but it has selected 3 papers which are not mine in my account. i want to delete that information. please help me

30 July 2024 3,743 0 View

What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?

29 July 2024 7,714 4 View

Zaib Ullah

Zoom is best option. As we all use nowaday for online classes.

Sudhir KS Yadav

I am already using zoom app to take virtual class. I have slso good experience about zoom app. The drawback of it is every slot is of half hour, after that we have to reconnect.

Thanks

Dr Sudhir Yadav

Magdalena Day

Simon Stephens thanks!