RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

05 February 2021 292 3 View

How to use other researchers data set for our algorithm? should i cite or get permission?

Dear friends i have a query. i have to use a data which is a real time case study done by a canadian author. he has given data in repository. my question is when i use this data how should i cite...

01 February 2021 3,603 6 View

How can I prepare 300mg/L concentration of lead(II) nitrate ( Mol. Wt reffered is 331.21) incoorporated Luria-Bertani (LB) agar ?

I need to check the Heavy metal (lead) consumption assay using microbes at varying concentration in same media. ( 200mg/L to 2000mg/L).kindly help me to clear it.

19 January 2021 1,946 9 View

Interested in being a reviewer for Scheduling-optimization-meta heuristics?

Dear fellow researchers, I need a two to three non indian reviewers for the research area of Scheduling-optimization-meta heuristics-operation research. all the journals are asking for other...

22 November 2020 9,633 5 View

What do you suggest to investigate cell signaling with the small number of cells?

I have a plan to investigate cell signaling on NKG2D-expressing CD4. For this matter, I plan to isolate primary cells from patients and use western blot technique to measure the expression level...

08 August 2020 911 5 View

Can anyone share UMATHT subroutine?

I want to see the format of formulation in your code, so i can write for my own problem. U, DUDT, DUDG(NTGRD), FLUX(NTGRD), DFDT(NTGRD), DFDG(NTGRD,NTGRD)

04 August 2020 5,268 4 View

What is the C45 concrete damage plasticity parameters for abaqus ?

Since I am preparing my fyp, but really don't how to find the value of C45(eurocode) to run ABAQUS to check RC column.... Then, I am first to use this software, please help... Thanks a lot..

02 June 2020 6,604 7 View

How to do rat TMJ dissection and do micro-CT for condyle?

Hello, everyone! I want to ask how to remove TMJ from rat's head? Is there a protocol or instruction? And, if I want to do micro-CT for TMJ of rat, can I scan the whole head or after removal will...

22 March 2020 8,708 4 View

Can there be a positive correlation In the following situation?

The results showed low expression of A and overexpression of B in Tumor tissues. We expect them to show a negative correlation, but they show a significant positive correlation. Can you explain...

06 March 2020 3,149 11 View

How to move my works from scientific research phase to commercialized phase ?

Radiochemistry dosimeters for radiotherapy and high dose applications

20 February 2020 4,444 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View