Does anyone have experience using High Molecular Weight DNA Extraction Kits for extracting DNA from plant leaves?

Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...

03 January 2021 9,487 4 View

Which software should a researcher working on continuous gas lift optimization use? PROSPER (PETEX) or PIPESIM (Schlumberger)?

I want to know which of the two softwares will be handy considering working on continuous gas lift optimization.

14 January 2020 8,901 2 View

Is it possible to estimate the biomass of a crop knowing only the yield per acre per year?

I am trying to estimate the carbon input (of vegetal residue) to an agricultural land, based on the historical records of the yield. However, the yield (lb per acre) is the only information I...

14 April 2019 7,370 3 View

Has anybody used Oligofectamine for co-transfection of siRNA and Plasmid DNA?

I am planning to co-transfect siRNA and Plasmid DNA and I have only Oligofectamine with me. Though Oligofectamine is recommended only for siRNA transfection I was wondering if Plasmid DNA can also...

17 April 2017 8,129 2 View

Do murine isolated NK cells in culture need to be trypsinized for passage or are they floating?

05 October 2016 1,652 3 View

Does anyone know a protocol for XL-10 gold competent cell preparation?

I'm trying to clone a 12.5 kb plasmid and I need competent cells of this strain.

22 January 2016 2,769 2 View

Does anyone have a good company for ordering lentiviral GFP vectors?

Does anyone have a good source/company for ordering lentiviral GFP vectors to be infected into tumor cells, tail vein injected into mice and then FACS sorted for GFP?

22 November 2015 3,227 2 View

Why I don't see heterochromatic enrichment of methylation in meDIP data from plants?

Hello, I have recently used meDIP to detect genome-wide methylation levels. After data analysis, I do not see the expected methylation-enrichment in heterochromatic regions. Any idea what may...

06 December 2014 7,271 3 View

How should I analyze metabolites from cell culture media using Mass Spectrometry?

I need to check whether the level of metabolites are altered in cell culture media during specific treatment condition because of altered uptake and secretions.

30 January 2014 2,766 11 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

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01 March 2021 3,905 5 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

01 March 2021 3,325 4 View