Nothing
in my opinion, directly no because the shape of the resin after performing mechanical stirring will be mixed up and maybe it gets a circular shape but then we can modify the shape ( by some...
21 February 2022 7,387 2 View
Hello everyone, I need CASIA-Iris V4 dataset for reimplementation of a paper as my final project. This is the link to the website:...
26 November 2021 5,201 0 View
Hi, I am doing RNA extraction from human PBMC (of 1.5 ml blood) using Trizol Reagent. I got rations A260/280 1.8~1.95 and A260/230 1.7~2.2 and the concentration about 150~360 ng/ul. I have tried...
20 June 2019 3,337 6 View
I am doing RNA extraction using Trizol Reagent (RiboEx). I got rations A260/280 1.60~1.90 and A260/230 0.5~1.50 and also low yield about 70~160 ng/ul. I want to know if using liquid nitrogen and...
31 May 2019 7,237 5 View
I have written sth brief about the interpretation of our results, and mentioned some limitations of the study... but what else to say? I don't know what to say, and how to talk about the rest of...
20 March 2019 1,079 6 View
802.11ah simulation matlab code
15 January 2019 7,416 1 View
, Water Quality Modelers
28 December 2014 3,617 4 View
If we couple two 1D maps of the form x(k+1)=g(x(k)), and get a 2D map like x(k+1)=f(y(k),x(k)); y(k+1)=f(x(k),y(k)), where the function f and its related parameters are the same for both...
18 May 2013 6,727 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View