CBC + Reticulocytes + Ferritin (out of febrile or inflammatory disease)+ IEF/HPLC Hbins with HbA2 dosing

ex : HbA2 > 3,5% + no iron deficit + mild microcitic anemia

Thank you for your answer, but I am mostly looking for a method that does not require complex tests and can be easily possible in the short time in areas with limited laboratory facilities

hemogram+HbA2 dosing (taking chgm N, as no iron deficit) ? sorry, for not helping too much - I'm clinician, but may I put you in connection with some lab exp ? best regards

Thank you very much for your useful instructions, this work needs more studies to be used practically everywhere, especially in deprived areas that have few laboratory facilities.

A cheap and easy field test is single tube osmotic fragility test at 0.36% buffered NaCl concentration. If you can calculate % fragility my measuring absorption at 540 nanometer wavelength, you will get better objective results. Or else, you can d0 "NESTROFT" an acronym used for Naked Eye Single Tube Red cell Osmotic Fragility Test. You will find plenty of literature on internet.

Hi, thalassemia carrier screening is one of the most widely performed genetic tests screening, so, one of the new test for prenatal detection of thalassemia is possible through non-invasive prenatal testing(NIPT), a simple and painless procedure that tests the fetus’s DNA through a maternal blood sample, NIPT is routinely used for chromosomal aneuploidies as it provides the expecting parents with information early, reassures them, and allows them to make informed decisions.

Ramesh Z. Patel

Thank you very much for your helpful advice

You are welcome

Thank you very much.

Also, a few MicroRNAs can use for β-thalassemia early recognition.

Gholamreza Shafiee

Thank you very much for your useful instructions, this work needs more studies to be used practically everywhere, especially in deprived areas that have few laboratory facilities.

In covid-19 epidemic condition,pcr and genomic techniquies are be increased in very countries and cities.

Cell blood count (CBC) with PBS analysis along side with measurement of HbA2 is the best and accessible way that you can screen the patients for beta thalassemia.

Thank you very much for your answer, doctor.