Hi there,

According to this paper: http://www.ncbi.nlm.nih.gov/pubmed/7793070/

TEV is fully active in the presence of NaCl up to 2M.

Thank's for the paper :)

My experience is use low salt or TEV is very inactive. The lower the better, even adding it after dialysis into low salt buffer increases yield of cleaved protein. It also is quite inactive in Imidazole as well so best to get rid of that as well.

Hi

As You can see TEV activity was not affected by 1M NaCl. The cleavage with TEV (3mg/1l of cell culture) occurred overnight in cold room during dialysis to remove 300mM imidazole.

1. Before TEV

2. After TEV

Best

Leszek

Hi leszek,

Can you elaborate more about your protocol, I am going to do the same with TEV?

Thanks

Source of a cheap nuclease is here: http://www.protean.cz/en/recombinant-protein/11/endonuclease-s-marcescens

Thanks Ladislav but it is not related to the topic.