24 Questions 61 Answers 0 Followers
Questions related from Haitham Tartor
Hi guys, I have an experiemnt where three groups of fish were used, two of them were treated with two doses of virus, while the thrid is a control group. I want to compare the means of gene...
07 July 2019 3,664 5 View
I will purify my r-protein (produced in E.coli) and cleave the MBP and His-tag off it and I have heard about the inclination of the r-protein to aggregate/precipitate when the tag cleavage takes...
03 March 2017 5,602 1 View
I am producing a recombinant protein in Rosetta, my protein is fused to MBP+his-tags at the amine terminus. I have solubilized it with sarkosyl. I am using IMAC column to purify my protein and...
03 March 2017 1,225 9 View
is it necessary to remove the sarkosyl- my r-protein is solubilized in sarkosyl- before mice immunization?
03 March 2017 6,681 2 View
My r-protein (in e.coli) is only solublized in urea and I want touse it in a downsteam immunization procedure, dentaured protein is not convenient for production of Ab, how to solve this problem ?
03 March 2017 9,142 2 View
I am producing my MBP+his-tagged r-protein in Rosetta (75 kDa). I am using the anti his-tag column t purify my protein. I collected the sample flow-through after passing through the columns in...
02 February 2017 3,896 12 View
I have a question regarding to your Amylose resin kit https://www.neb.com/products/e8021-amylose-resin I read in the protocol...
02 February 2017 9,607 7 View
Hi guys, I want to ask about RNA isolation procedures from fish skin tissue samples? a full protocol would be appreciated.
03 March 2016 5,189 3 View
Is it tricky to use MS to sequence a small recombinant portion of salmon IgT?
01 January 2016 2,185 0 View
I have a question regarding the result of MS (protein sequencing). We made the recombinant r- IgT (unique immunoglobulin in fish) in mammalian HEK cells, we want to characterize the r-protein, we...
12 December 2015 6,005 6 View
Do you know If the urea is quite more efficient than SDS as a protein denaturant? I always experience a smear in my SDS-PAGE and I thought Urea may help me in getting rid of this smear.
11 November 2015 4,126 14 View
Ask: I want to share the results of my experiment to confirm the transfection rate of hek 293 cells (suspended not adherent) with a plasmid that reports with GFP, 2 days and 5 days after...
10 October 2015 2,550 5 View
Hi guys, I am going to conduct an affinity purification experiment using a synthetic peptide coupled to an agarose matrix to pick up the specific Abs from the pAb fro my rabbit serum, my question...
10 October 2015 5,555 3 View
problem of mucus and epithelial cells when isolating skin leukocytes?
10 October 2015 8,141 0 View
I conducted an affinity purification experiment. I tested the wash for any unbound Ab and I found a signal in the 0,5 M NaCl wash, and I want to incubate it again with the matrix to catch as much...
10 October 2015 1,184 5 View
Usually with IHC protocol, I wash before and after H2O2 with TBS buffer, recently I found some protocols use dH2O instead of the buffer, which one is better?
09 September 2015 9,469 3 View
how to solve the problem of transmembrane protein smearing in SDS and western blot ?
08 August 2015 3,943 0 View
I am using hek 293 mammalian system for flag and strept tagged secreted IgT production, I used direct blotting method and I got a smear in SDS-PAGE with a very faint expected size of my protein in...
08 August 2015 6,933 15 View
What is the kit with the easiest protocol implementation that I can buy to disconsolate my r-IgT before SDS-PAGE to avoid smear.
08 August 2015 7,373 0 View
I want to measure the concentration of protein in given mucus samples , the problem is that during sample collection a lot of water may comes in yyour samples which makes your sample diluted and...
08 August 2015 10,012 8 View
Please, If anybody could help, we are producing r-IgT from fish in mammalian system and we got a smear on SDS-PAGE, we send parts of gel smear to the MS, but unfortunately results are quite...
08 August 2015 4,990 3 View
I am using Novagen kit for r-protein purification and I have a smear in my SDS -PAGE after purification, do I need a post purification treating of my r-protein for long storage ??
07 July 2015 5,315 6 View
Would you mind recommending me a book about recombinant protein production in mammalian system from A to Z , I want to produce IgT.
01 January 2015 8,068 2 View
Iunintentionally, I have used 1 micro gram , which is know to be too much, of my PET-M41.IgT plasmid to transform JM109 e.coli strain, and that eventually results in colonies on LB-kanamycin...
01 January 1970 9,617 7 View
