Tetanus toxin uptake in neurons?

20 September 2020 0 7K Report

We have tetanus toxin heavy chain fragment C (TTHc). It is suppose to have the ganglioside binding site for uptake. We can provide fluorescent TTHc. I am looking for someone who can show retrograde up of the protein in the neuron

Badges
Science topic

More Andrew Lees's questions See All

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,690 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,291 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,333 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,084 6 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

Cresyl violet Nissl Staining ?

Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...

18 July 2024 2,420 0 View

The Uniqueness of Human Language?

Unlike humans, it is believed that birds do not have a symbolic language system that can be reduced to words (Berwick et al. 2012). But they do have a nervous system that can generate sequences...

14 July 2024 9,852 0 View

Differentiation of KOLF-NGN2 hiPSC into neurons - Troubleshooting?

Hello everyone, We are trying to differentiate the KOLF-NGN2 hiPSC into neurons - A reference human induced pluripotent stem cell line for lar... . The differentiation is characterized by 2...

09 July 2024 4,002 1 View

Where to source glass rings compatible with cell culture?

Hi all, I am looking glass cilinders that have an open flat bottom and top. Preferably with the inner diameter in the range of 2 cm. It's similar to the ones found on multi-electrode arrays for...

06 July 2024 5,846 1 View

I'm stimulating the shaffer collateral but do not want to generate LTP, what herz am I limited to?

I'm a graduate student at UWM focusing on electrophysiology. I'm trying to perform Shaffer Collateral stimulation of CA1 pyramidal neurons. If I were to have a protocol with 6s of no stim, 2...

18 June 2024 319 0 View

Fully Connected Neural Network ?

How to modify a neuron in the fully connected layer individually and design it as a statistical indicator such as kurtosis or negative entropy，as shown in the figure

16 June 2024 7,299 0 View

How to simulate crossbar architecture of SNN?

I can understand that we can use Sentaurus TCAD for designing neuron and synapse. But to implement full crossbar architecture using these neuron and synapse for image classification application,...

16 June 2024 6,879 0 View

Why the rat cortical primary neurons suddenly dead/turn into bad condition after about 6days?

I used SD rats (E19) for cortical neuron extraction. Due to the need for subsequent special experiments, I cultured the neurons in a Corning PDL 384-well plate. The culture medium is Neurobasal +...

12 June 2024 1,714 4 View