Among various factors, the temperature is one of the most crucial factor for enzyme activity. For most of the enzyme assay, researcher use 37oC or normal room temperature as incubation temperature. If we consider the poikilothermic animals like fish, it's natural enzyme activity depends on habitat temperature and each fish (tropical, temperate, polar or cold water species) has their optimum temperature at which it grows best and we know growth is nothing but a consequence of optimum (good) metabolism.
So, is it right to use the mentioned assay temperature for enzyme activity of fish irrespective of its natural habitat?
Or,
Do the researchers need to modify the assay temperature according to optimum natural habitat temperature of fish?
Please provide your suggestions.
We may not adopt a single temperature for fish enzymes such as 37ºC in case of human samples. It should be specific the species as fish is not a single species. Again, we need to find optimum temp for each of the enzymes as well. For convenience, we can select a constant temperature for all enzyme assays, which can be selected based on the optimum temperature for growth of that species. In the following publication, you will find an optimum range of temperature for digestive enzymes, however its quite a wide one (45-65ºC) https://scialert.net/fulltext/?doi=jfas.2017.264.272#:~:text=The%20optimum%20temperatures%20for%20both,water%20temperature%20of%20the%20swamp.
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