Telomere length measurement by qPCR: which protocol is better - Cawthon 2002 or Cawthon 2009?

06 June 2013 1 4K Report

It seems to me like the 2009 method is preferable, but many recent papers use the 2002 protocol. Is there any reason for this?

Badges
Science topic

More Kerry Andrea Pettigrew's questions See All

Methylation analysis relating to cognitive traits - does DNA have to be from the tissue of interest?

Wondering whether the use of DNA from saliva (or non-tissue-specific DNA in general) is generally considered to be acceptable for methylation analysis. I'm interested to look at this within the...

01 February 2014 9,372 5 View

Designing qPCR assay to validate a 3Mb CNV (sybr green or equivalent)

How many primer pairs would I need to design to validate this size of CNV by qPCR? Would one PCR fragment be enough, or would I need several?

04 May 2013 2,759 0 View

Has fine mapping by sequencing the region flanking an associated SNP been rendered obsolete by recent developments e.g. 1000 Genomes?

I am wondering if people are still resequencing regions flanking an associated SNP, in order to identify hitherto undiscovered common variants which may be the causative SNP? Or is this a bit of...

10 November 2012 613 3 View

What's better for DNA extraction, phenol chloroform or salting out?

I'm more used to extracting DNA using spin column kits, but trying to work out what is the best non-kit option, in terms of yield and DNA quality.

09 October 2012 4,534 8 View

I have (Linkage Disequilibrium) LD data for two SNPs - r2 is about 0.14, D' is around 0.8. Could these SNPs be said to be in strong LD?

Is it better to cite D' or r2 values when considering LD?

08 September 2012 306 35 View

What is the earliest age that a child has been diagnosed with type 1 diabetes?

I'm wondering how early diabetic complications could potentially begin, in terms of neonatal brain development. Could the effects of poor glucose regulation begin immediately after birth, and how...

08 September 2012 4,762 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View