It seems to me like the 2009 method is preferable, but many recent papers use the 2002 protocol. Is there any reason for this?
Cawthon 2002 is simple than Cawthon 2009.
Wondering whether the use of DNA from saliva (or non-tissue-specific DNA in general) is generally considered to be acceptable for methylation analysis. I'm interested to look at this within the...
01 February 2014 9,372 5 View
How many primer pairs would I need to design to validate this size of CNV by qPCR? Would one PCR fragment be enough, or would I need several?
04 May 2013 2,759 0 View
I am wondering if people are still resequencing regions flanking an associated SNP, in order to identify hitherto undiscovered common variants which may be the causative SNP? Or is this a bit of...
10 November 2012 613 3 View
I'm more used to extracting DNA using spin column kits, but trying to work out what is the best non-kit option, in terms of yield and DNA quality.
09 October 2012 4,534 8 View
Is it better to cite D' or r2 values when considering LD?
08 September 2012 306 35 View
I'm wondering how early diabetic complications could potentially begin, in terms of neonatal brain development. Could the effects of poor glucose regulation begin immediately after birth, and how...
08 September 2012 4,762 2 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View