Kerry Andrea Pettigrew

7 Questions 6 Answers 0 Followers

Questions related from Kerry Andrea Pettigrew

Methylation analysis relating to cognitive traits - does DNA have to be from the tissue of interest?

Wondering whether the use of DNA from saliva (or non-tissue-specific DNA in general) is generally considered to be acceptable for methylation analysis. I'm interested to look at this within the...

02 February 2014 9,407 5 View

Telomere length measurement by qPCR: which protocol is better - Cawthon 2002 or Cawthon 2009?

It seems to me like the 2009 method is preferable, but many recent papers use the 2002 protocol. Is there any reason for this?

06 June 2013 3,709 1 View

Designing qPCR assay to validate a 3Mb CNV (sybr green or equivalent)

How many primer pairs would I need to design to validate this size of CNV by qPCR? Would one PCR fragment be enough, or would I need several?

05 May 2013 2,816 0 View

Has fine mapping by sequencing the region flanking an associated SNP been rendered obsolete by recent developments e.g. 1000 Genomes?

I am wondering if people are still resequencing regions flanking an associated SNP, in order to identify hitherto undiscovered common variants which may be the causative SNP? Or is this a bit of...

11 November 2012 640 3 View

What's better for DNA extraction, phenol chloroform or salting out?

I'm more used to extracting DNA using spin column kits, but trying to work out what is the best non-kit option, in terms of yield and DNA quality.

10 October 2012 4,565 8 View

What is the earliest age that a child has been diagnosed with type 1 diabetes?

I'm wondering how early diabetic complications could potentially begin, in terms of neonatal brain development. Could the effects of poor glucose regulation begin immediately after birth, and how...

09 September 2012 4,797 2 View

I have (Linkage Disequilibrium) LD data for two SNPs - r2 is about 0.14, D' is around 0.8. Could these SNPs be said to be in strong LD?

Is it better to cite D' or r2 values when considering LD?

09 September 2012 342 35 View