Which anesthetic for cortisol determination in fish?

I am a bit confused. I am planning an experiment in the field where fish will be caught by electrofishing. Afterwards, they are supposed to be euthanised by using an anaesthetic. I found...

10 February 2021 1,101 4 View

What is the first thing that comes to your mind when you think of the effectiveness of foreign aid on development? Effective/not effective, why?

I'm currently doing a research project on the effectiveness of U.S. aid in Georgia, and would very much appreciate your insights.

22 December 2020 4,189 1 View

Why do lentiviral vectors not express my protein when I infect cells with them?

I use pWPI plasmid to overexpress two proteins in neurons. I did it once, and everything worked beautiful. But now I am not capable of overexpressing the proteins, even tough I use the same...

09 December 2020 4,036 4 View

Can peptides survive high temperatures?

I'm looking for ways to functionalise a material with a cell-adhesive peptide (relatively short chain - 6aa) and I was just wondering if peptides can withstand high temperatures (100-150 C) for 30...

15 January 2020 8,978 4 View

Abnormally high fluorescence read with qpcr, and no amplification showing on agarose gel, what could be going on?

Hi! I've been running qPCR on some drinking water samples with a primer/ probe FAM assay. In any given run, there have been at least a few samples with artificially high fluorescence reads,...

16 October 2019 8,060 3 View

How to prepare agar amended with mercuric chloride?

Several studies mention using agar amended with mercuric chloride when isolating mercury resistant bacteria. Is there a lab method available for preparation of these plates? If no, what are some...

17 June 2019 5,047 5 View

Interview guides for UTAUT?

30 January 2019 5,443 4 View

How much does our childhood affect the way we think and make investment choices?

Subconscious, subjective drivers.

22 January 2019 5,397 5 View

ReNcell VM cell culture problems - why are they dying after I split them?

The cells look healthy after thawing, but they die when I try to split them. I have tried making fresh media and fresh matrigel, and have tried filtered and non-filtered Accutase. Does anyone have...

19 December 2018 630 9 View

Is it necessary to dechorionate zebrafish embryos to extract genomic DNA and RNA ?

I tried to dechorionate embryos with pronase at 1mg/mL. I made 3 washes but I obtained a high mortality rate and the medium was dirty. Thank you for your answers

03 January 2017 3,455 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View