Hi, I am looking for sequencing my soil samples. My objective to study bacterial diversity in the response of nitrogen fixation. Could you suggest me the good target gene and primers. I am wondering to go with MiSeq (2*250) technique. Thanks..
NifH and NifD genes! you may get database as well as literature on these.
131
Hi Manoj
You can read this reference1
http://www.microbiologyresearch.org/docserver/fulltext/micro/150/5/1301.pdf?expires=1490072155&id=id&accname=guest&checksum=F319889E81DBDFAA4716976543965315
2
https://jb.asm.org/content/196/3/595.full
3
https://onlinelibrary.wiley.com/doi/10.1111/j.1574-6941.2008.00545.x/pdf
4
https://www.utsc.utoronto.ca/~fulthorpe/qiannifH.html
5
Good Luck
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