Manoj K. Sekhwal

5 Questions 6 Answers 0 Followers

Questions related from Manoj K. Sekhwal

Does incubation period influence 15N2 isotope results

Hi, I tried 15N2 method to determine free living bacterial activity in soil samples at 2d, 4d and 8d incubation period. However, i have did not find considerable activity. Does incubation period...

04 July 2017 1,283 4 View

Free living Nitrogen fixation determination by 15N2 isotope?

Hi, I am trying to determine free living N fixation in soil by 15N2 isotope. I tried with several protocols at different amount of doubled labeled 15N2. I used different parameters, However, i did...

05 April 2017 2,073 1 View

Target gene and primer in bacteria for nitrogen fixation ??

Hi, I am looking for sequencing my soil samples. My objective to study bacterial diversity in the response of nitrogen fixation. Could you suggest me the good target gene and primers. I am...

18 March 2017 8,784 8 View

Does nitrogen fixation formation in soil effects if jars are flushing by different gas (e.g. Nitrogen, Helium, Argon) ?

Hi, Does nitrogen fixation formation in soil effects if jars are flushing by different gas (e.g. Nitrogen, Helium, Argon) ? if so could advice which is good gas for flushing jars to make as much...

01 February 2017 5,589 2 View

Is samples required immediate oven dry after remove incubation of 15N2?

Hi, We are following 4days incubation of 15N2 into 20mL sealed vials. Could advise, is samples required immediate oven dry after remove incubation of 15N2 or vials can put open for some of the...

01 February 2017 3,206 0 View