Dear researchers

I am working on tacrolimus nanoparticle and when trying to determine the E.E. of the tac using HPLC ,it shows 2 peaks for the tac and its tautomers I , although I tried to change the solvents the peaks stay ,

How can I measure the area?

May I sum the two peaks areas and consider it as one peak for the tac concentration measurements?

thanks

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