How can I design an experiment capable of demonstrating if my cells can grow without a substrate?

Dear Researchers, I am incredibly confused about the in vitro colony forming assay. I was asked to perform such assay to assess my cell models capability to grow without attachment to a solid...

10 June 2020 6,230 3 View

How do I convert a list of smiles to Name in MOE?

I have a MOE database with molecule codes and smiles that I have to convert to name. What is the easiest way to do this? Thank you.

04 May 2020 6,987 3 View

Do you know how to release bacteria after adhesion to epithelial cells?

11 January 2018 7,377 6 View

LEXSY L. tarentolae growth problems. tips?

Hi! Anyone working with L. tarentolae LEXSY protein expression system? Despite having used the protocol described by Jena I am having trouble on parasite growth. Any further tips you might have?

03 April 2016 5,658 3 View

What is the correct reagent for (transfection) Cytomix?

KHPO4, K2HPO4 and/or KH2PO4?

31 December 2014 6,356 3 View

Can anyone help me with primer design for biomarker research?

I'm trying to determine a strategy to study the expression of certain parasites' biomarkers in different conditions. Firstly, I do not know if the best approach would be to start with common PCR...

25 March 2014 3,863 3 View

Can anyone help me with primer design for biomarker research?

I'm trying to determine a strategy to study the expression of certain parasites' biomarkers in different conditions. Firstly, I do not know if the best approach would be to start with common PCR...

02 March 2014 1,418 4 View

Experimental or quasi-experimental?

I am currently reading one paper which randomly assigned students of four classes for experimental and control conditions. However this randomization process was within class and not in a whole,...

01 January 1970 1,538 3 View

How can I peel off PDMS from an acrylic mold with a micropattern?

I'm trying to make micropatterns on acrylic to use it as a mold for PDMS. The first time I tried this, PDMS got stuck to the micropattern area of acrylic, even though I made a silanization to the...

01 January 1970 809 19 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What is the optimal method to prepare a controlled porosity osmotic pump (CPOP) tablet for scanning electron microscopy?

In order to assess the shape and size of pores formed by dissolving the pore former in the semipermeable membrane structure, SEM images are needed. Cellulose acetate was used in the coating...

15 February 2021 6,135 3 View

Professional editing and proofreading services?

08 February 2021 7,901 6 View

How to calculate random forest manually?

17 January 2021 301 2 View

Is there and standard of Chemical price when writing a SCI paper?

Hello I writing a SCI paper and want to make a table for chemical price comparison. However, the chemical price varies from country and company, amount and also dates. Is there any reference...

12 January 2021 5,600 1 View

How do we solve optimization problems involving a maxima or minima in the objective function ?

I am trying to solve a multi-objective optimization problem using the Pyomo library in Python. The objective function includes operational optimization of an electrolyzer for hydrogen production...

11 January 2021 8,018 3 View

Why nan loss values are resulted through deep learning in python?

hello every one i want to practice deep learning as descibed for a home price prediction problem i use code as following # IMPORTING PACKAGES import pandas as pd # data processing import numpy...

07 January 2021 8,761 1 View

Can you help with the implementation of two different model of deep learning for stock market prediction?

I have stock price data(Open, high, low, close), I want to do prediction by combining two different models like CNN with others. Dataset will be same, what are the ways that I can use for...

04 January 2021 1,845 3 View

I'm just learning the ePrime 3.0 software and have created a test. How can I put this test on a tablet for subjects to use for data collection?

This is probably a really basic question, but I can't figure it out. I've loaded the test on a USB and transferred to the tablet, but am unable to get it to run. Any help would be wonderful. Thank...

23 December 2020 5,202 3 View