I have proceeded to extract whole protein from human monocyte derived dendritic cells, using regular lysis with RIPA lysis buffer followed by centrifugation to obtain soluble protein suspension fractions. However, I had considerable poorer protein yields than I normally have with tumour cells using the same protocol. Does anyone have any tips to increase extraction yields besides increasing the amount of starting material?

I am considering adding a mechanical disruption step with a needle or even snap freeze the cell suspension, but I’m not sure that will be enough. Any tips?

Thank you in advance.