How to validate regridding results of environemental data to higher spatial resolution in R?

I am working with several continous, environmental marine datasets at varying original spatial resolutions. My aim is to regrid each dataset to one common, high resolution grid. I am currently...

22 February 2021 2,290 2 View

How to do a Repeated measures analysis with not normal distributed data?

I have results from a listening test in 12 sound environments which are build up out of 3 room properties and 4 occupancy rates. Since the sound samples were often intelligible, subject scores...

04 November 2020 5,423 11 View

Can I use my DNA after the tube was "sterilized" by UV light?

I left tubes with isolated DNA drying (before resuspension) in a biosafety hood for too long and a colleague came and turned on the UV light for some 20-30min. The light did not shine on the DNA...

29 September 2020 536 3 View

How could I avoid mutations upon transforming my gRNAs into the lentiCRISPRv2 vector?

After I have cloned my gRNAs into the lentiCRISPRv2 vector and sent the construct for sequencing, I have noticed that a single mismatch between my clone and original sequence has been introduced....

11 September 2020 4,066 1 View

DNA extraction of swabs stored in ethanol?

Hi all, I am using buccal swabs of seals as a sample source for microsats and mitochondrial sequencing. There is a few samples that were shipped stored in ethanol. , i.e. swab of the buccal area...

08 September 2020 1,013 1 View

How do I interprete the results of a moderation analysis with a categorical and a continuous variable?

Hi all, I have a question concerning a moderation analysis within an experimental setting. The independent variable is categorical and has two options. The moderator is a continuous variable. I...

06 January 2020 9,511 1 View

How to run a binary logistic regression with two experimental treatments?

Hi all, is it possible to run a binary logistic regression within an experiment? That means that the two experimental treatments (treatment 1 vs. control) predict a categorical variable...

03 December 2019 7,319 9 View

What method should I use to freeze the human kidney tissue samples?

Thehuman kidney tissue samples will be used later for immunohistochemistry, ELISA, gene analysis, etc. Should I freeze it in solution or not?

20 June 2019 3,052 3 View

How do THP-1 cells normally look like after PMA treatment?

We just started to culture THP-1 cells and treat them with PMA (100nM) for 72 h. Afterwards we change the media daily as recommended. Does anyone have experiences with serum-free culture in the...

23 October 2017 4,566 1 View

How do I understand the output of my covariate in the Repeated measures ANOVA design?

Dear Leard team, I am currently experiencing some issues with my Master thesis data analysis and I would appreciate your help!!! First I will explain you my study design and then I will explain...

25 April 2017 4,467 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View