Hi all,
I recently performed NEGATIVE selection of human T cells from PBMCs with BD iMag T cells enrichment kit. Although the purity is high, I noticed that the cells are activated (no matter the negative selection) and eventually 24h after the separation they undergo cell death. Any ideas/suggestions to avoid this? The cells were cultured in 6-well plate for 24h (for 24h-downstream applications and no interest in further culture) in complete RPMI medium.
Thanks!
3 problems when using classical magnetic separation rack in cell sorting
Biomagnetic cell separation is an alternative to centrifugation, columns, filtration and precipitation. It eliminates undue cell-stress and reduces the risk of negative impact on cell function and phenotype.
Sorting cells from a heterogeneous population enables the study of the different isolated types, but also allows for the introduction of enriched cell populations to a patient. The use of highly selective separation procedures is also critical to improve cell-based treatments on stem cell therapy, tissue engineering and regenerative medicine.
If you are interested in cell isolation with biomagnetic separation, download our newest guide Does it matter which magnetic separation rack I use in my cell isolation processes?:
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