Spyridoula Tsiomita

4 Questions 2 Answers 0 Followers

Questions related from Spyridoula Tsiomita

CFSE proliferaton analysis in BD DIVA software?

Hi all, is there a way to analyze cell proliferation with CFSE with BD DIVA software? I activate murine splenocytes or purified CD4 and I use Cell Trace and follow the exact protocol but I only...

16 March 2021 8,458 1 View

T cells magnetic separation troubles?

Hi all, I recently performed NEGATIVE selection of human T cells from PBMCs with BD iMag T cells enrichment kit. Although the purity is high, I noticed that the cells are activated (no matter the...

31 May 2018 439 1 View

Best tool for finding dna binding sites between a known dna sequence and possible targets?

For all bioinformatic experts, I have a known sequence (DNA) and I'd like to find the DNA-binding consensus seq. between my known seq. of interest and possible others (unknown) targets. Which is...

14 October 2015 5,250 3 View

How can I do a Competitive ELISA set up from scratch?

I'd like to perform a competitive ELISA from scratch. Being a first-timer, I would appreciate if someone could help me with the basic concetrations of Ab and antigen in oder to start with and...

06 April 2015 9,738 2 View