Hello Salma,

Yes, it should be enough.

Full activation of T cell requires the cell to receive and integrate signals from a minimum of three diverse types of receptors:

A. The T-cell receptor (TCR, signal 1),

B. Costimulatory receptors (signal 2), and

C. Cytokines (signal 3).

Each of these signals elicits biochemical signaling cascades and provides information to the T cell to help shape the generation of effector T cells.

The stimulation can be of two kinds namely,

1) antigen dependent in which case you will require an antigen and co-stimulation from antigen presenting cells and

2) antigen independent in which case you will not need an antigen but a mitogen like PHA or anti-CD3 and anti-CD28 antibodies.

Cytokines such as IL-2 have their role to play as a growth and survival factor capable of inducing T cell proliferation.

Best.

I recommend to look up the work of Federica Sallusto and Antonio Lanzavecchia, where they use blood-derived DCs to stimulate T cells from the same donor with antigen. Not sure whether just adding the viral peptide to the PBMC culture would work.

Dear Hisse,

Could you give us the complete reference for 'Federica Sallusto and Antonio Lanzavecchia'?

Thank you!

Thomas M.C. Binder I tink this was the first paper where Sallusto and Lanzavecchia used monocyte-derived DCs to stimulate T cells:

doi: 10.1084/jem.179.4.1109.. 1994 Apr 1;179(4):1109-18.J Exp Med

PMID: 8145033

Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha

Federica Sallusto and Antonio Lanzavecchia

Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukin 4 and downregulated by tumor necrosis factor alpha.

doi: 10.1084/jem.179.4.1109.. 1994 Apr 1;179(4):1109-18.J Exp Med

PMID: 8145033

https://rupress.org/jem/article/179/4/1109/25035/Efficient-presentation-of-soluble-antigen-by