Synchronize cells before chemosensitivity and proliferation assays?

More Aristeidis E. Boukouris's questions See All

Which are the best ways to analyze the data of PM2.5 & PM10 in the atmosphere collected by gravimetric analysis?

14 April 2024 3,953 3 View

Can someone indicates me a database about meteorological variables per country?

I'm looking for variables like solar irradiation, wind speed, humidity, temperature and precipitation

06 April 2024 1,926 2 View

How to validate numerical results of back to back placed cold formed channel sections?

Currently I am working on the numerical analysis of back to back placed cold formed channel sections with different cfrp wrapping configuration. How can I validate the shear analysis of my...

28 March 2024 3,709 1 View

Mitosox in mitochondrial extract?

Hi everyone, I was wondering if someone has a protocol for using MitoSOX in mitochondrial-enriched extracts. MitoSOX can migrate to the nucleus and generate an inaccurate response about...

24 March 2024 466 0 View

What is the correlation between schizophrenia and criminal behavior among adults?

Despite of certain psychiatric conditions that could increase a person's risk of committing a crime, research suggests that patients with mental illness/disorder may be more prone to violence if...

11 March 2024 996 2 View

How can I solve ABAQUS warnings like negative eigen values, displacement too big for contact and numerical singularity?

Currently I am working on numerical validation of the paper 'Laboratory and numerical analysis of steel cold formed sigma beams retrofitted by bonded CFRP tapes - extended research' (Laboratory...

25 February 2024 6,380 1 View

How can I add a CFRP layer on top and bottom of flange in abaqus?

I am doing an analysis of bending of cfrp wrapped I section in abaqus and I need to provide cfrp on top and bottom of flanges, using which tool can I provide the cfrp, I am facing issues due to...

22 February 2024 7,639 2 View

Two-way anova analysis with no interaction effects but pairwise comparisons true?

Hi there. I have a problem with my research. I conducted a two-way ANOVA analysis (2x2). There is no interaction effect of the two independent variables on the dependent one (p=0.062), but the...

11 January 2024 7,090 5 View

How does the equipment optimization done in Formaldehyde Production Plant?

for my 2nd last year of university, I got a project to design equipment for the formaldehyde plant and I had to mention the inlet and outlet condition of every piece of equipment. With the 2 weeks...

09 December 2023 9,546 0 View

I have requested the article The Impact of AI Chatbot-Based Learning on Students’ Motivation in English Writing Classroom. when & where will I get it?

That is my question.

21 November 2023 6,740 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View