How does one interpret polynomial quadratic regression of two variables ?

01 February 2019 4,995 8 View

How to carry out quality control using Trimmomatic on SRA reads of SAGE-RNASeq?

I have carried out a FASTQC analysis on raw reads of SAGE-RNASeq experiment from SRA database. Numerous overrepresented sequences have turned up pertaining to Illumina single end adapters and...

11 December 2016 8,201 0 View

Improving RAM usage of GNU parallel blast?

Running a GNU parallel blast uses only ~2% of my memory per core. My system has a quad-core 24 GB RAM. 1.Can I affect the speed of blast process and reduce the time of run by alloting more memory...

08 September 2016 8,304 2 View

How can I use a proxy with authentication details for R or Rstudio?

I am using R 3.2 and Rstudio in Windows 7. Since, I am using a proxy internet connection, I always get the 407 proxy authentication error. I have tried setting environment variables with my proxy...

01 February 2016 6,558 2 View

Computational requirements to assemble a small mammalian genome (~600 Mb)?

Please provide a minimal advisable requirement of RAM and CPU Cores. Also, provide the time (in hours or days) required per such assembly, if experienced.

01 February 2016 8,591 9 View

Is it possible to predict long non-coding RNAs without a reference genome?

01 February 2016 2,496 2 View

What are the most intriguing questions in the field of non-coding RNA studies?

The field of non-coding RNA research is one of the quickly emerging areas in the world of science. Few years ago, we had just tRNA and rRNA; then suddenly we had ribozymes and now we have the...

09 October 2015 7,131 1 View

How do grid cells get the message that the individual has ventured into a new location in space?

How does a grid cells get the information?

01 February 2015 5,411 4 View

How can we add chemical residues to the pdb structure of an RNA molecule in In silico mode?

I've to add a methyl group to the C-5 of a Uracil base in the 3D structure of an RNA molecule. Please suggest tools to do so.

06 July 2014 2,328 4 View

Can anyone help me set up a high performing cluster running on Ubuntu Linux in my laboratory with 128 Gb RAM, 32 Core processor and 1 TB memory?

I have two Quad core HP Z400 workstations and two quad core Acer Veriton series with i3 processor.

04 May 2014 5,863 16 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View