Suitable fluorescent dye/dye to visualise ocular injection location?

03 November 2023 1 8K Report

I'm trying to identify a marker/dye that can be assessed (via histology) from post-mortem eyes which have undergone subretinal and suprachoroidal injections. Any advice on successful agents would be useful.

Refik Kanjhan

Dear Andrew,

Years ago I used to use 5% Pontamine Sky Blue (in 0.5 M sodium acetate) dye in vivo in rat brains to label the location of extracellular unit recording sites. You will need to apply 5-10 microAmp currents for about 10 minutes. You can visualize permenant dye deposits as blue dots in at the injection. It is relatively cheap and easy to visualize with a light microscope. For details of the methods please see Methods section in the following publication.

best wishes,

Refik

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