tapping mode tips are too stiff to measure extracellular matrix roughness. I am looking to find the right cantilever type with a stiffness (k-value) that is able to do so in solution.
For ExtraCellular Matrix samples I would use PeakForce QNM (Hybride Mode or similar) with contact mode cantilever (e.g. soft Ultra-Short Cantilevers from Nanoworld are good candidates)