Suggestions for reliable, ethanol resistant lab markers?

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Most accurate way to determine IC50 values by flow cytometry?

We are trying to use flow cytometry to detect cell viability / apoptosis to determine IC50 values after exposing adherent cells to drugs. We are concerned that during the washing / processing...

23 June 2024 4,279 0 View

Protecting Sensitive Maternal Health Information in Baby's Record?

Mother's mental health condition was included in cut and paste over to baby's chart and released without her authorization.

13 March 2024 3,362 0 View

What information do you have on the following: the purpose and application of the comparative method in linguistics, role in determining proto forms?

I have an essay to write on the comparative method in linguistics I have to discuss the purpose and application of the comparative method in linguistics, emphaising its role in protoforms, state...

27 February 2024 7,681 0 View

How to avoid the high background of PBS (the control of solvent) during Nanoparticle Tracking Analysis (NTA) of exosomes?

Dear colleagues, Has anyone of you ever experienced difficulties with the high background of the solvent-PBS (used to suspend the exosomes after isolation by ultracentrifugation) during NTA...

09 January 2024 7,735 3 View

Had research been done on restarting hormone replacement therapy (HRT) after some years off it?

All approved medical treatments for osteoporosis have risks associated with them. Has anyone actually researched the idea of restarting HRT after patients have tried other therapies?

23 November 2023 4,719 0 View

Can anyone reccommend a GST antibody to tether recombinant proteins to a chip for SPR?

Cytiva sells a GST antibody as part of a rather expensive kit for coupling tagged proteins to their sensor chips. The antibody can not be purchased separately, and we do not need all the other...

26 October 2023 3,800 1 View

What is the signficance of shape changes/peak height in a protein thermal melt curve?

I have been performing thermal melt analysis of a protein that can form dimers or other higher order structures. Thus, we were not too surprised to see a melting profile that seems to have two...

22 October 2023 9,445 0 View

2 IVs with 4 groups and 2 DVs continuous - test??

I have two IVs with 4 groups each and two DVs that are continuous. What statistical test do I use? Thank you.

20 July 2023 157 4 View

Best method to activate/expand cryopreserved naive T cells?

I've been trying to activate and expand naive T cells that have previously been isolated from PBMCs with a pan-T cell isolation kit from Miltenyi and cryopreserved in 90% FBS 10% DMSO. I have...

10 July 2023 9,088 2 View

Does anyone know what became of Albert Mascall Devine, a leading scholar on Alexander the Great in the 1980's and '90's?

I have recently managed to have Devine's Master's thesis on Curtius posted on the UQ espace, but access is limited to current students and staff. I'd like to see it made more widely available,...

16 November 2022 5,875 7 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?

12 July 2024 9,904 1 View

Quelles applications innovantes peuvent bénéficier des propriétés des géopolymère?

Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...

10 July 2024 7,457 1 View

Do you know about fully Ethanol toyata Inova car?

https://youtu.be/5MckkqF9XWU?si=MvBsElJZg8_At8qP

09 July 2024 6,038 5 View

Is gasohol or Ethanol petrol mixture safe , ch?

What is safe gasoline and Ethanol safe or Ethanol petrol mixture safe for car fuelin.

03 July 2024 2,939 3 View

What is alternative for PMMA?

If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...

03 July 2024 2,371 1 View

How can we reduce the porosity of tungsten oxide (WO3) thin films during DC sputtering deposition (target: W, power: 100 W, 75 W, and 50 W)?

We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...

01 July 2024 2,686 2 View

Hi i m workiking with hct116 resistant and naïve cells after passage half of my cells die can anybody help?

thanks

25 June 2024 4,667 0 View