Can the mass spec of a protein digest be relevant to a protein characterization?

I have characterized a protein using cDNA cloning of its mRNA. I also have digested the protein using MALDI-TOF mass spec. Ideally I would have like to have done MALDI-TOF tandem MS, but it was...

09 October 2014 8,990 3 View

How do I set up a phylogenetic tree?

09 October 2014 8,507 10 View

Why do PCR products have to be incorporated into plasmids and copied by bacteria to be sequenced?

Why can't we just sequence PCR material directly? There should be many more copies of the pcr fragment than anything else.

09 October 2014 1,130 19 View

Suggestions for a journal to submit to?

I have characterized the myoglobin of quail using cDNA cloning and now need to find a journal to submit to. Suggestions would be great. Impact factor not that important but not completely obscure....

09 October 2014 3,300 0 View

What is the role of cysteine residues in myoglobin?

Among mammals, humans have a rare cysteine residue. Avian myoglobins also seem to lack this AA. I wish to understand what role a cysteine residue may play on stucture and/or function of the...

08 September 2014 481 9 View

Why do some peptide peaks fail to resolve in MALDI-TOF?

Please list reasons why peaks sometimes don't show up, even if they should theoretically be there. I also have many unknown peaks that I know may be contaminants but could they also be something...

08 September 2014 8,176 6 View

How can we predict the protein sequence from the DNA of another organism?

I have a project where I need to theorize the protein sequence of quail myoglobin. A very similar organism (chicken) has already had its myoglobin sequenced. I can access the entire genome of...

05 June 2014 3,981 6 View

Why are my bands be below the appropriate marker in SDS-PAGE?

I have done a myoglobin extraction using salting in/out, dialysis, and size exclusion columns. When running the extraction on SDS PAGE against a low MW ladder I get the following results (pic...

05 June 2014 412 6 View

How can I use the NCBI database to design primers?

I have the mRNA sequence for myoglobin in quail. But I cannot figure out how to use this to find the dna sequence for the gene so that I can design primers for it. The BLAST website is still fresh...

05 June 2014 8,868 7 View

Do I need a special imaging system for 2D gels?

We have great document scanners already and are using Coomassie to stain the gels. Would this be adequate for imaging software if the scanners have high enough dpi?

04 May 2014 9,847 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View