I have characterized the myoglobin of quail using cDNA cloning and now need to find a journal to submit to. Suggestions would be great. Impact factor not that important but not completely obscure. Thank you.
I have characterized a protein using cDNA cloning of its mRNA. I also have digested the protein using MALDI-TOF mass spec. Ideally I would have like to have done MALDI-TOF tandem MS, but it was...
09 October 2014 9,088 3 View
Why can't we just sequence PCR material directly? There should be many more copies of the pcr fragment than anything else.
09 October 2014 1,220 19 View
I am trying to set up a phylogenetic tree in MEGA 4.0. I have the coding myoglobin DNA sequences for two types of quail, chicken, turkey, and duck. I'm guessing neighbor joining method is best?...
09 October 2014 8,615 10 View
I have characterized the myoglobin of quail using cDNA cloning and now need to find a journal to submit to. Suggestions would be great. Impact factor not that important but not completely obscure....
09 October 2014 3,385 0 View
Among mammals, humans have a rare cysteine residue. Avian myoglobins also seem to lack this AA. I wish to understand what role a cysteine residue may play on stucture and/or function of the...
08 September 2014 628 9 View
Please list reasons why peaks sometimes don't show up, even if they should theoretically be there. I also have many unknown peaks that I know may be contaminants but could they also be something...
08 September 2014 8,306 6 View
I have a project where I need to theorize the protein sequence of quail myoglobin. A very similar organism (chicken) has already had its myoglobin sequenced. I can access the entire genome of...
05 June 2014 4,101 6 View
I have done a myoglobin extraction using salting in/out, dialysis, and size exclusion columns. When running the extraction on SDS PAGE against a low MW ladder I get the following results (pic...
05 June 2014 675 6 View
I have the mRNA sequence for myoglobin in quail. But I cannot figure out how to use this to find the dna sequence for the gene so that I can design primers for it. The BLAST website is still fresh...
05 June 2014 8,978 7 View
I was wondering why many in-gel digestion protocols suggest making fresh buffer. I am using ammonium bicarbonate at 25 mM and 100 mM for a Glu C reaction (resources cannot pinpoint the best...
04 May 2014 3,619 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.
06 August 2024 6,796 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
As authors and academic writers, we usually prepare our manuscript using our own resources. We submit these manuscripts to scientific Journals for peer review and publication sometimes at a fee....
03 August 2024 7,304 2 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
I am an Mphil research student and looking for high impact factor battery related journals to download and read research articles to improve my knowledge and get new ideas in the field of battery...
01 August 2024 6,516 2 View