13 Questions 10 Answers 0 Followers
Questions related from John Goodson
I have characterized the myoglobin of quail using cDNA cloning and now need to find a journal to submit to. Suggestions would be great. Impact factor not that important but not completely obscure....
10 October 2014 5,463 0 View
10 October 2014 3,359 0 View
I have characterized a protein using cDNA cloning of its mRNA. I also have digested the protein using MALDI-TOF mass spec. Ideally I would have like to have done MALDI-TOF tandem MS, but it was...
10 October 2014 9,055 3 View
Why can't we just sequence PCR material directly? There should be many more copies of the pcr fragment than anything else.
10 October 2014 1,192 19 View
I am trying to set up a phylogenetic tree in MEGA 4.0. I have the coding myoglobin DNA sequences for two types of quail, chicken, turkey, and duck. I'm guessing neighbor joining method is best?...
10 October 2014 8,578 10 View
Among mammals, humans have a rare cysteine residue. Avian myoglobins also seem to lack this AA. I wish to understand what role a cysteine residue may play on stucture and/or function of the...
09 September 2014 599 9 View
Please list reasons why peaks sometimes don't show up, even if they should theoretically be there. I also have many unknown peaks that I know may be contaminants but could they also be something...
09 September 2014 8,269 6 View
I have a project where I need to theorize the protein sequence of quail myoglobin. A very similar organism (chicken) has already had its myoglobin sequenced. I can access the entire genome of...
06 June 2014 4,063 6 View
I have done a myoglobin extraction using salting in/out, dialysis, and size exclusion columns. When running the extraction on SDS PAGE against a low MW ladder I get the following results (pic...
06 June 2014 625 6 View
I have the mRNA sequence for myoglobin in quail. But I cannot figure out how to use this to find the dna sequence for the gene so that I can design primers for it. The BLAST website is still fresh...
06 June 2014 8,938 7 View
I was wondering why many in-gel digestion protocols suggest making fresh buffer. I am using ammonium bicarbonate at 25 mM and 100 mM for a Glu C reaction (resources cannot pinpoint the best...
05 May 2014 3,592 1 View
We have great document scanners already and are using Coomassie to stain the gels. Would this be adequate for imaging software if the scanners have high enough dpi?
05 May 2014 9,908 4 View
I am working on a project of sequencing a myoglobin protein. Well I managed to get my SDS page bands digested at a facility near me and mass spec'd and fingerprinted, and the results, while good,...
01 January 2014 4,732 1 View
