Mainly, I am working for prevention of sugarcane diseases by methods of mutagenesis. For that, I am using two types of mutagens , one is chemical mutagen and other is physical mutagen. In case of chemical mutagen, Ethylmethane sulfonate is being used and other case, I am confusion which radiation is used weather UV radiation or gamma radiation. In all cases, I have chosen callus for treatment. Would you please suggest me looking my above question, what would be better gamma or UV radiation? My next question is, After treating the callus, what would be my genetic analysis steps? or an other things , if you want to suggest ? please suggest me?