Mitochondria can sense and control nutrient catabolism and anabolism and they are becoming ever so important in cancer biology.
Apart from the in vitro techniques involving FACS, TEM, metabolomics, enzyme assays and Seahorse what are some other ways to show mitochondrial dysfunction. More importantly, how do we show this in animal models.
Dear Md Wasim!
Please You look at folowing articles:
Mouse models for mitochondrial diseases
https://academic.oup.com/hmg/article/25/R2/R115/2198134
http://genesdev.cshlp.org/content/23/15/1714.full
Article Mitochondrial Dysfunction in Genetic Animal Models of Parkin...
You can use fluorescence microscopy to study mitochondrial membrane potential. Usually mitochondria are very sensitive to cellular stress and any dysfuntion in mitochondria will lead to change in mitochondrial membrane potential (either depolarization leading to apoptosis, or hyper polarization). In either case you can use TMRM assay or other similar assay to visualize changes in mitochondrial membrane potential. It might suffice your need!
Good luck!
Akshay
Seahorse, mitotrackers (green and red, for dysfunction probably red better), by flow cytometry and confocal microscopy, and other mitochondrial probes for confocal microscopy should be a good proof of concept. At molecular level are different molecules involved in mitochondrial function and biogenesis: TFAM, NRF1, MYC, TUFM and others that could has aberrancies in expression or signaling with mitochondrial dysfunction.
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