Hi
I am going to tow bio molecules with bioorthogonal reaction. Inverse electron demand diels–alder reaction is very faster than Strain-promoted Azide-Alkyne Cycloaddition . What is advantage of Strain-promoted Azide-Alkyne Cycloaddition over the inverse electron demand diels–alder? Are Tetrazine and (E)-Cyclooct-4-enol stable in aqueous solution?
The huisgen cycloaddition has become very popular in in vitro and in vivo experiments due to it's quick reaction in physiological conditions and pH. Both azide- and cyclooctyne-containing compounds are well tolerated in cell cultures. Reaction times are usually
Thanks
Is your mean Strain-promoted Azide-Alkyne Cycloaddition ?
Why is it more popular than Inverse electron demand diels–alder reaction?
Yes, the huisgen cycloaddition is a strain-promoted azide-alkyne reaction: https://en.wikipedia.org/wiki/Azide-alkyne_Huisgen_cycloaddition
It's very popular because the simplicity of synthesis of reactants and also the bio-compatibility of the reaction: https://en.wikipedia.org/wiki/Copper-free_click_chemistry
I know that. But I'm confused which way I choose in my project. Inverse electron demand diels–alder reaction is very fast.
Are you work on the strain-promoted azide-alkyne reaction in lab?
I don't see many references to the use of diels-alder reactions in vivo or on living tissue, which isn't surprising since reaction conditions are usually relatively harsh.
We use azide-alkyne cycloaddition on live tissue for transplantation with no issues.
Thank you very much for your guidance.
How long did it take to complete the reaction?
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