I want to make a stock solution of polyA for use in my assay but I cannot find any information about how to store it once it is solubilized. The solution will be made by solubilizing polyadenylic acid potassium salt in water.
Dear Sally, I have stored aliquots of aqueous poly[A] (as well as poly[C], [G] and [U]) solution in the -20oC freezer for months with no obvious reduction of activity in my assay. Avoiding multiple freeze-thaw cycles is always advisable, so make small aliquots and mark usage on the lid. I guess it depends what your assay is. Will a gradual reduction in length be detrimental or not?
Good luck,
A.
Dear Sally, I have stored aliquots of aqueous poly[A] (as well as poly[C], [G] and [U]) solution in the -20oC freezer for months with no obvious reduction of activity in my assay. Avoiding multiple freeze-thaw cycles is always advisable, so make small aliquots and mark usage on the lid. I guess it depends what your assay is. Will a gradual reduction in length be detrimental or not?
Good luck,
A.
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