Dear all,
It is not clear to me what to do if I want to freeze at -20°C some tissue samples in RNAlater. I read that it is recommended to first leave at 4°C the sample in RNAlater overnight. After that, should I remove the tissue from the RNAlater solution and freeze it, or should I keep the sample in RNAlater and freeze it?
Also, if someone has experience with such procedure (RNAlater at -20°C), if he/she had good recovery of RNA, or if it is better to freeze at -80°C.
Thank you very much
I place tissue samples in RNAlater at 4ºC for a couple of days and then move the tubes to a -80ºC. I find this to be the best way to ensure longer-term survival of nucleic acids. Storage in -20ºC also works, but nucleic acids may not fare that well in the long run, i.e. >1-2 years.
See also https://www.ncbi.nlm.nih.gov/pubmed/17202763
https://www.ncbi.nlm.nih.gov/pubmed/17202763
Hi Sergios, thank you very much for this paper, very interesting! From the paper it seems that it doesn't make a difference if removing or keeping the RNAlater when moving to -80°C (after placing in RNAlater at 4°C). What do you usually do in your case? Do you remove the RNAlater solution when moving to -80°C. I am wondering if this makes a difference also in practical terms. Thank you
Hi Niccolo , what was your experience , did u removed or kept the RNA later while moving it to -80.
Thank you very much
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