Niccoló Alfano

4 Questions 4 Answers 0 Followers

Questions related from Niccoló Alfano

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

11 February 2021 3,857 4 View

Tools to understand changes in function/structure of a protein caused by non-synonymous substitutions?

Hi everybody, I found a strain of a virus with few non-synonymous substitutions compared to the reference sequence. Can please anyone suggest me a tool to understand if those changes amino acids...

02 October 2019 3,144 3 View

Storage of tissue samples in RNAlater at - 20°C: do I need to keep the samples in RNAlater solution or should I remove it?

Dear all, It is not clear to me what to do if I want to freeze at -20°C some tissue samples in RNAlater. I read that it is recommended to first leave at 4°C the sample in RNAlater overnight. After...

18 May 2017 1,409 3 View

In which cases is it necessary to treat the cDNA with RNase H?

I have been reading several publications where reverse transcription was performed and I have seen that in many cases RNase H is used after reverse transcription and during second strand...

08 February 2017 4,081 4 View