Using glycerol to store anaerobic bacteria is the same as aerobic bacteria? I use the original glycerol to store aerobic bacteria at a ratio of 1:1. I wonder if the process is the same when storing anaerobic bacteria as aerobic bacteria?
Ways to store anaerobic bacteria?
Kelechukwu Nwokorie
To properly preserve anaerobic bacteria, anaerobic conditions must be maintained during the growth, harvesting, dispensing, and freezing processes. The cryoprotectant and suspending medium must also be pre-reduced, and anaerobic conditions should be maintained with oxygen-free gas flow using a sterile cannula. This is also done through polymeric encapsulation.
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